Transformation of Somatic Embryos of Soybean With Chitinase and Beta-1,3-Glucanase Genes via Particle Bombardment
Chanprame, Sontichai
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https://hdl.handle.net/2142/85053
Description
Title
Transformation of Somatic Embryos of Soybean With Chitinase and Beta-1,3-Glucanase Genes via Particle Bombardment
Author(s)
Chanprame, Sontichai
Issue Date
1998
Doctoral Committee Chair(s)
Widholm, Jack M.
Department of Study
Crop Sciences
Discipline
Crop Sciences
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Genetics
Language
eng
Abstract
Chitinase, maize and bean chitinase and maize $\beta$-1,3-glucanase gene were transformed into somatic embryos of soybean using the conditions described above. Enzyme activity assays indicated that the transformed progeny had higher activity than that of the wild type and homozygous progeny had higher activity than heterozygous progeny. Northern analysis indicated that the transgenic progeny of line 26 and 29 produced the 1.1 kb mRNA for bean chitinase. The progeny from line 29 also produced an mRNA which was larger than normal size. Pathogenic assays suggested that expression of the transgene could enhance disease resistance to brown stem rot, sudden death syndrome and brown leaf spot at the early stage of plant and disease development.
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