Modification of Vegetable Soybean Sugar Content via Gene Transformation

Chanprame, Sermsiri

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Description

Title

Modification of Vegetable Soybean Sugar Content via Gene Transformation

Author(s)

Chanprame, Sermsiri

Issue Date

1998

Doctoral Committee Chair(s)

Widholm, Jack M.

Department of Study

Crop Sciences

Discipline

Crop Sciences

Degree Granting Institution

University of Illinois at Urbana-Champaign

Degree Name

Ph.D.

Degree Level

Dissertation

Keyword(s)

Agriculture, Plant Culture

Language

eng

Abstract

To modify soybean sugar content, somatic embryos of soybean cvs. Peterson Jade, Daelip Hakja (PI 157.419), Yakumo Meaka (PI 417.468), Kinshu (PI 549.065) Kaohsiung No.1 (PI 536.546) and Jack were transformed with yeast invertase (SUC 2) and $\alpha$-galactosidase (agl A) from Aspergillus niger under the control of the lectin promoter using particle bombardment. The transformation was not very successful. In some cultivars, no green clumps were obtained after 8 weeks of selection in FG medium containing 25 $\mu$g/ml hygromycin B. The green clumps obtained from transformation and selection were subjected to PCR analysis for the transgenes. Some lines were PCR positive for the hygromycin resistance and/or $\alpha$-galactosidase genes but Southern and northern analysis gave negative results. These results suggest that the transformed genes were lost during the somatic embryo multiplication due to the friability of some green clumps that were discarded during the culture process.

Graduation Semester

1998

Type of Resource

text

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