Expression and Localization of Foreign Proteins in Soybean and Tobacco Seed Tissue
Philip, Reena
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https://hdl.handle.net/2142/85051
Description
Title
Expression and Localization of Foreign Proteins in Soybean and Tobacco Seed Tissue
Author(s)
Philip, Reena
Issue Date
1997
Doctoral Committee Chair(s)
Vodkin, Lila O.
Department of Study
Crop Sciences
Discipline
Crop Sciences
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Agriculture, Agronomy
Language
eng
Abstract
Another objective of this research was to test a transformation procedure for soybean with an expression vector containing a casein gene, under the control of the seed-specific lectin promoter. Soybean is one of several crop plants which are still difficult to transform even in the hands of plant transformation specialists. Transformation of soybean embryogenic suspension cultures was achieved via microprojectile bombardment method. Several independently transformed clones were obtained, but the problems of regenerating these clones into a plant are still be to be solved. Genomic southern blot analysis of these transgenic clones shows multiple integration events of the introduced genes.
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