Effects of Heterodera Glycines Population Densities on Fusarium Solani F. Sp. Glycines Colonization of Soybean Roots and Development of Sudden Death Syndrome

Jackson, Tamra A.

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https://hdl.handle.net/2142/85020 Copy

Description

Title

Effects of Heterodera Glycines Population Densities on Fusarium Solani F. Sp. Glycines Colonization of Soybean Roots and Development of Sudden Death Syndrome

Author(s)

Jackson, Tamra A.

Issue Date

2005

Doctoral Committee Chair(s)

Niblack, Terry L.

Department of Study

Crop Sciences

Discipline

Crop Sciences

Degree Granting Institution

University of Illinois at Urbana-Champaign

Degree Name

Ph.D.

Degree Level

Dissertation

Keyword(s)

Biology, Microbiology

Language

eng

Abstract

Estimation of root colonization by Fsg with the plating bioassay was difficult and the results were often unsatisfactory in these experiments. Therefore, an alternative method was explored, relative quantification with real-time quantitative PCR (QPCR), to estimate fungal colonization of soybean roots. QPCR was more sensitive than the plating bioassays at detecting the fungus: Fsg DNA was detected in 371 out of 372 root samples with QPCR, while CFU were only detected in 14.5% of the samples with the plating bioassay. The plating bioassay required at least 10 days longer to complete and the results had higher variability than those obtained with QPCR. Overall, QPCR had many advantages over the plating bioassay and has the potential for several more practical applications.

Graduation Semester

2005

Type of Resource

text

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http://hdl.handle.net/2142/85020

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