Global Expression Profiles and Targeted Pathway Modulation During Soybean Embryo Development
Thibaud-Nissen, Francoise
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https://hdl.handle.net/2142/85013
Description
Title
Global Expression Profiles and Targeted Pathway Modulation During Soybean Embryo Development
Author(s)
Thibaud-Nissen, Francoise
Issue Date
2003
Doctoral Committee Chair(s)
Vodkin, Lila O.
Department of Study
Crop Sciences
Discipline
Crop Sciences
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Agriculture, Agronomy
Language
eng
Abstract
In the second approach, five CHS transgenic soybean lines were generated. Relative mRNA abundance of transgenic and endogenous CHS genes was measured by real-time PCR. Three lines showed low transgene expression but increased transcript abundance for two endogenous CHS genes One line showed a 10-fold increase in CHS expression over the control seeds, but no change in isoflavone content, and is a candidate for more extensive profiling of flavonoid compounds.
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