A Genomic Assessment of Vicia Villosa Roth: Evaluation by Flow Cytometry, Canonical Discriminant Analysis, and Replicated cDNA Microarray Analysis

Yeater, Kathleen Mary

Permalink

https://hdl.handle.net/2142/85011 Copy

Description

Title

A Genomic Assessment of Vicia Villosa Roth: Evaluation by Flow Cytometry, Canonical Discriminant Analysis, and Replicated cDNA Microarray Analysis

Author(s)

Yeater, Kathleen Mary

Issue Date

2003

Doctoral Committee Chair(s)

Bollero, German A.

Department of Study

Crop Sciences

Discipline

Crop Sciences

Degree Granting Institution

University of Illinois at Urbana-Champaign

Degree Name

Ph.D.

Degree Level

Dissertation

Keyword(s)

Agriculture, Agronomy

Language

eng

Abstract

The final research objective is to determine statistically significant expressed genes in hairy vetch that are also biologically significant to traits hypothesized to be integral in hairy vetch improvement. The RNA of four targets were hybridized to 142 cDNA clones of biotic stress genes and gene sequences of soybean that were robotically spotted onto aminosilicated slides. The microarray data was analyzed using the individual fluorescence intensities to fit two statistical models with a mixed model analysis of variance using Proc Mixed in SAS. Ninety-seven of the 142 gene sequences (68%) were differentially expressed in at least one of the target comparisons. Eighty-three of the sequences (58%) were differentially expressed in hairy vetch. Among these are two sequences that encode for proline-rich proteins, which are abundant in cell wall structures and are involved in plant development.

Graduation Semester

2003

Type of Resource

text

Permalink

http://hdl.handle.net/2142/85011

Owning Collections