University of Illinois Urbana-Champaign

Modulation of the Expression of Multidrug Resistance Proteins by Sulforaphane and Erucin

Harris, Kristin E.

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Description

Title
Modulation of the Expression of Multidrug Resistance Proteins by Sulforaphane and Erucin
Author(s)
Harris, Kristin E.
Issue Date
2007
Doctoral Committee Chair(s)
Elizabeth Jeffery
Department of Study
Nutritional Sciences
Discipline
Nutritional Sciences
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Health Sciences, Nutrition
Language
eng
Abstract
Sulforaphane increases gene expression through binding of the Nrf2 transcription factor to the antioxidant response element (ARE) in the promoter region of target genes. Because SF and ER increased MRP1 expression at the mRNA level, we investigated the impact of these compounds on MRP1 transcription. Non-small cell lung cancer cells (A549) and human liver carcinoma cells (HepG2) were transfected with an MRP1-luciferase reporter gene and treated with SF or ER. Neither SF nor ER increased luciferase activity in the transfected cells. In addition, overexpression of Nrf2 in A549 cells had no effect on MRP1-luc expression. These results indicate that SF and ER increase MPR1 mRNA and protein levels via a non-transcriptional mechanism and that activation of Nrf2 does not induce transcription of the mrp1 gene.
Graduation Semester
2007
Type of Resource
text
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http://hdl.handle.net/2142/84951

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