Short-Chain Fatty Acids and Functional Adaptations in the Neonatal Intestine
Albin, David Miller
This item is only available for download by members of the University of Illinois community. Students, faculty, and staff at the U of I may log in with your NetID and password to view the item. If you are trying to access an Illinois-restricted dissertation or thesis, you can request a copy through your library's Inter-Library Loan office or purchase a copy directly from ProQuest.
Permalink
https://hdl.handle.net/2142/84936
Description
Title
Short-Chain Fatty Acids and Functional Adaptations in the Neonatal Intestine
Author(s)
Albin, David Miller
Issue Date
2004
Doctoral Committee Chair(s)
Kelly A. Tappenden
Department of Study
Nutritional Sciences
Discipline
Nutritional Sciences
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Health Sciences, Human Development
Language
eng
Abstract
Intestinal adaptation following massive loss of absorptive surface area is an important process that results in enhanced intestinal function to compensate for reduced absorptive capacity. Short-chain fatty acids (SCFA) are known to enhance this adaptive process in adult animal models, and data from our lab indicates that butyrate (Bu) results in marked changes in glucose transport in the intestine within minutes. We hypothesized that SCFA and Bu would enhance glucose transport and transporter gene expression in a neonatal pig model of massive small bowel resection. Two-day-old neonatal pigs underwent 80% jejunoileal massive small bowel resection, and were fed via total parenteral nutrition (TPN). Piglets were randomized to receive the following treatments: (1) control TPN; and isoenergetic, isonitrogenous TPN supplemented with; (2) SCFA (36 mM acetate, 15 mM propionate, 9 mM Bu, for a total of 60 mM SCFA); (3) 9 mM Bu (9Bu); or (4) 60 mM Bu (60Bu). Piglets were further randomized to examine acute (4 h, 12 h, 24 h) and chronic (3 d, 7 d) adaptations. With SCFA and Bu treatments, improvements in glucose, amino acid, and dipeptide transport were observed in the proximal small intestine (duodenum and jejunum), but not ileum. Transport of certain nutrients (glucose and arginine) was enhanced in the colon by SCFA and Bu. Whenever effects were observed with the SCFA group, similar effects were also observed with Bu group(s), indicating that Bu is the main SCFA responsible for these changes. Using a microarray approach with isolated jejunal epithelia, 9Bu was found to affect expression of nearly one-third of the >10,000 total genes examined. This included induced expression of several nutrient transporter genes, indicating that Bu enhances expression of nutrient transporters in the intestinal epithelia. In addition, studies examining peptide transporter (PepT1) protein indicated that 9Bu induces trafficking of PepT1 to the brush-border membrane. Therefore, Bu enhances intestinal adaptation in a neonatal pig model, probably by increasing transcription of nutrient transporters, as well as increasing trafficking of specific transporters to the brush-border membrane. Butyrate offers great promise as a nutritional therapy for neonatal short-bowel syndrome patients.
Use this login method if you
don't
have an
@illinois.edu
email address.
(Oops, I do have one)
IDEALS migrated to a new platform on June 23, 2022. If you created
your account prior to this date, you will have to reset your password
using the forgot-password link below.
