Engineering Enhanced Cytochromes P450: Understanding Thermostability and Mechanism Through Rational Design and Directed Evolution
Maves, Shelley Ann
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https://hdl.handle.net/2142/84922
Description
Title
Engineering Enhanced Cytochromes P450: Understanding Thermostability and Mechanism Through Rational Design and Directed Evolution
Author(s)
Maves, Shelley Ann
Issue Date
2001
Doctoral Committee Chair(s)
Sligar, Stephen G.
Department of Study
Biochemistry
Discipline
Biochemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Molecular
Language
eng
Abstract
Although random and rational approaches for protein engineering are often used as separate approaches, this work has demonstrated the benefits of combining these approaches. Through the use of both methods a better understanding of thermostability, catalytic mechanism and substrate specificity was gained.
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