DNA and Ligand Binding and Kinetic Properties of Wild-Type and Mutant RsrI [n6-Adenine] DNA Methyltransferases
Szegedi, Sandra Suzanne
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https://hdl.handle.net/2142/84915
Description
Title
DNA and Ligand Binding and Kinetic Properties of Wild-Type and Mutant RsrI [n6-Adenine] DNA Methyltransferases
Author(s)
Szegedi, Sandra Suzanne
Issue Date
2000
Doctoral Committee Chair(s)
Gumport, R.I.
Department of Study
Biochemistry
Discipline
Biochemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Language
eng
Abstract
To complement the biochemical characterization of M•Rsr I in vitro, DNA binding analysis in vivo was performed using the bacteriophage P22-based challenge-phage assay. I isolated a catalytically impaired mutant, M•RsrI (L72P), that, unlike wild-type M•RsrI, binds in a methylation-sensitive, site-specific manner to DNA.
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