The Estrogen Response Element and the KRAB Repression Domain as Modulators of Estrogen Receptor Action
De Haan, Georgius
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https://hdl.handle.net/2142/84908
Description
Title
The Estrogen Response Element and the KRAB Repression Domain as Modulators of Estrogen Receptor Action
Author(s)
De Haan, Georgius
Issue Date
1999
Doctoral Committee Chair(s)
Shapiro, David J.
Department of Study
Biochemistry
Discipline
Biochemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Language
eng
Abstract
Repressing expression of estrogen regulated genes would greatly facilitate studies of their function. Therefore we transformed ER into a transcription repressor by fusing the KRAB transcription repressor domain of the Kox1 protein to ER and various ER mutants. Incorporation of DBD mutations conferring increased ERE affinity previously identified in a genetic selection, allowed us to effectively repress in a ligand dependent fashion both basal and estrogen receptor induced transcription from a promoter containing a single non consensus ERE. These ER-KRAB chimeras therefore constitute powerful tools for the study of estrogen regulated gene expression.
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