University of Illinois Urbana-Champaign

Selection and Characterization of a Steroid Receptor DNA Binding Domain-Based Repressor of Estrogen Response Element Containing Promoters

Chusacultanachai, Sudsanguan

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https://hdl.handle.net/2142/84903

Description

Title
Selection and Characterization of a Steroid Receptor DNA Binding Domain-Based Repressor of Estrogen Response Element Containing Promoters
Author(s)
Chusacultanachai, Sudsanguan
Issue Date
1999
Doctoral Committee Chair(s)
Shapiro, David J.
Department of Study
Biochemistry
Discipline
Biochemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Language
eng
Abstract
To generate a repressor of the ERE-containing genes, we dimerized one of the up-binding mutants, DBD5, with a flexible linker and fused it to a strong KRAB repressor domain. The dimerized FLAGDBD5-KRAB (FdDBD5K) is a much more potent repressor than the monomer FLAGDBD5-KRAB (FDBD5K). The FdDBD5K elicited significant repression of both constitutive and estrogen receptor induced transcription on a cERE containing promoter and on a natural pS2 promoter. The FdDBD5K chimera should be a useful new tool for controlling the transcription of ERE-containing promoters.
Graduation Semester
1999
Type of Resource
text
Permalink
http://hdl.handle.net/2142/84903

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Dissertations and Theses - Biochemistry