High Pressure Protein Denaturation: Description of a High Pressure Intermediate and Effects of Mutations on Pressure Stability
Bondos, Sarah Elizabeth
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https://hdl.handle.net/2142/84895
Description
Title
High Pressure Protein Denaturation: Description of a High Pressure Intermediate and Effects of Mutations on Pressure Stability
Author(s)
Bondos, Sarah Elizabeth
Issue Date
1998
Doctoral Committee Chair(s)
Sligar, Stephen G.
Jonas, Jiri
Department of Study
Biochemistry
Discipline
Biochemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Language
eng
Abstract
Four mutations were also examined to determine how specific packing defects affect high pressure denaturation. Mutations were made in three positions in the core to determine how the position contributes to changes in stability. Also, charged and oversized residues were introduced into the core to determine how particular types of defects affect the pressure stability of the native and high pressure intermediate states. The effects of mutation are shown to be more dependent on the nature of the mutation, with charge burial mutations being the most deleterious given that all positions are buried in the AGH core.
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