University of Illinois Urbana-Champaign

Human Estrogen Receptor(alpha) Activity on Estrogen Responsive, Integrated Chloramphenicol Acetyltransferase (Cat) Reporter Constructs in Chinese Hamster Ovary (Cho) Cells

Eicken, Elizabeth Jean

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https://hdl.handle.net/2142/84890

Description

Title
Human Estrogen Receptor(alpha) Activity on Estrogen Responsive, Integrated Chloramphenicol Acetyltransferase (Cat) Reporter Constructs in Chinese Hamster Ovary (Cho) Cells
Author(s)
Eicken, Elizabeth Jean
Issue Date
1998
Doctoral Committee Chair(s)
Shapiro, David J.
Department of Study
Biochemistry
Discipline
Biochemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Molecular
Language
eng
Abstract
The estrogen receptors endogenous to CHO cells did not induce transcription of chromosomal copies of the more complex 2ERETKCAT promoter. In transient transfections hER$\alpha$ regulated transcription of the three 2ERETKCAT cell lines. We found that an ER mutant containing only AF1 elicited a dose dependent activation of the 2ERETKCAT promoter. This represents the first report of activation of an integrated reporter gene by this activation function.
Graduation Semester
1998
Type of Resource
text
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http://hdl.handle.net/2142/84890

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