High-Throughput Detection of Protein---Protein and Protein---Dna Interactions via Photonic Crystal Biosensors: Applications to Apoptosis Inducing Factor
Heeres, James T.
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https://hdl.handle.net/2142/84871
Description
Title
High-Throughput Detection of Protein---Protein and Protein---Dna Interactions via Photonic Crystal Biosensors: Applications to Apoptosis Inducing Factor
Author(s)
Heeres, James T.
Issue Date
2010
Doctoral Committee Chair(s)
Hergenrother, Paul J.
Department of Study
Biochemistry
Discipline
Biochemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Language
eng
Abstract
"High-throughput screening (HTS) has played an integral role in the discovery of lead molecules that eventually are transformed into the therapies of tomorrow. Contributing towards the success of any screening campaign is the target choice, chemical library size and diversity, as well as the technology utilized in screening. Photonic crystal (PC) biosensors are a relatively new technology whose potential is only beginning to be realized. PC biosensors are a ""label-free"" technology compatible with standard high-throughput techniques. Macromolecular interactions including protein---small molecule, protein---nucleic acid, protein---protein, and whole cell interactions can be measured using PC biosensors. Presented herein is the first application of PC biosensors to a protein---nucleic acid interaction and a simplified protein---protein interaction platform. The protein---nucleic acid interaction targeted is the apoptosis inducing factor (AIF)---DNA interaction, which is critical for the mediation of caspaseindependent cell death in neurodegenerative disease and stroke. Protein---protein interactions were targeted by modifying the PC biosensor surface for detection of the His6 tag, which is widely employed in protein purification."
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