The Use of High-Affinity T Cell Receptors to Study Specificity in the 2C T Cell System
Bowerman, Natalie A.
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https://hdl.handle.net/2142/84864
Description
Title
The Use of High-Affinity T Cell Receptors to Study Specificity in the 2C T Cell System
Author(s)
Bowerman, Natalie A.
Issue Date
2009
Doctoral Committee Chair(s)
Kranz, David M.
Department of Study
Biochemistry
Discipline
Biochemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Health Sciences, Immunology
Language
eng
Abstract
Finally, soluble high-affinity TCRs are promising reagents for the detection and diagnosis of specific virus or tumor antigens (peptide-MHC) on the cell surface. In addition, the TCRs would provide a useful probe for tracking the processing and presentation of specific antigens. Chapter 6 discusses the development of soluble single-chain TCRs with enhanced properties, such as increased avidity and signal amplification, to detect antigen. The results show that tetrameric and multi-fluorophore labeled forms of the high-affinity 2C TCR called m67 were capable of detecting low levels of specific peptide-MHC on the surface of both tumor cells and when the antigen was cross-presented on stromal cells in a solid tumor.
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