University of Illinois Urbana-Champaign

Biochemical Analysis of the Molecular Factors of Leucyl -Trna Synthetase That Ensure Aminoacylation Fidelity

Hellmann, Rachel Alice

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Description

Title
Biochemical Analysis of the Molecular Factors of Leucyl -Trna Synthetase That Ensure Aminoacylation Fidelity
Author(s)
Hellmann, Rachel Alice
Issue Date
2009
Doctoral Committee Chair(s)
Martinis, Susan A.
Department of Study
Biochemistry
Discipline
Biochemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Language
eng
Abstract
The canonical genetic code has potentiel to be expanded by developing orthogonal aaRS:tRNA systems. Tools to generate an orthogonal LeuRSaRNA Leu pair were developed by using a two plasmid system in an E. coli strain containing a nonfunctional genomic lacZ gene with an amber stop codon. The plasmid pRAH curies tRNALeu with an amber suppressor anticodon, while pKEG harbors LeuRS. From this system, directed evolution approaches can be used to screen for an orthogonal tRNA and a LeuRS derivative that will charge it.
Graduation Semester
2009
Type of Resource
text
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http://hdl.handle.net/2142/84863

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Graduate Dissertations and Theses at Illinois PRIMARY
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Dissertations and Theses - Biochemistry