Characterization of the Chemoreceptors in the Bacillus Subtilis Chemotaxis System
Glekas, George D.
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https://hdl.handle.net/2142/84859
Description
Title
Characterization of the Chemoreceptors in the Bacillus Subtilis Chemotaxis System
Author(s)
Glekas, George D.
Issue Date
2008
Doctoral Committee Chair(s)
Ordal, George W.
Department of Study
Biochemistry
Discipline
Biochemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Language
eng
Abstract
Physical characterization of the chemoreceptors was probed by identification of a zinc ion binding area, using isothermal titration calorimetry. In addition, in vivo chemotactic assays showed that altering the charges of the methylation sites on the chemoreceptors can have a significant effect on the activity of the receptor. Furthermore, individual methylation sites were shown to be responsible for both changes in the affinity of the chemoreceptor for attractant and receptor activation. These findings suggest a novel, more complicated role for methylation than previously suggested, and lead to a clearer picture of the coordination of the three adaptational systems in the B. subtilis chemotactic sensory transduction.
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