Mechanistic Investigations of Dehydration of Lacticin 481 Synthetase and Exploring Its Biochemical Applications
You, Young-Ok
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https://hdl.handle.net/2142/84858
Description
Title
Mechanistic Investigations of Dehydration of Lacticin 481 Synthetase and Exploring Its Biochemical Applications
Author(s)
You, Young-Ok
Issue Date
2008
Doctoral Committee Chair(s)
van der Donk, Wilfred A.
Department of Study
Biochemistry
Discipline
Biochemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Language
eng
Abstract
Developing a selective labeling method for peptides/proteins is of great interest in numerous research areas. In order to explore this idea, a strategy was developed through which an N-terminal ketone was introduced by using the LctA-LctM modification system. Finally, analysis of phosphorylated LctA intermediates in collaboration with Professor Kelleher's laboratory revealed dehydration of LctM takes place with directionality, moving from the N-terminus to the C-terminus of the substrates.
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