University of Illinois Urbana-Champaign

Biochemical and Structural Studies of the Enzymes Involved in RNA Cleavage and Modification

Cicmil, Nenad

This item is only available for download by members of the University of Illinois community. Students, faculty, and staff at the U of I may log in with your NetID and password to view the item. If you are trying to access an Illinois-restricted dissertation or thesis, you can request a copy through your library's Inter-Library Loan office or purchase a copy directly from ProQuest.

Permalink

https://hdl.handle.net/2142/84850

Description

Title
Biochemical and Structural Studies of the Enzymes Involved in RNA Cleavage and Modification
Author(s)
Cicmil, Nenad
Issue Date
2008
Doctoral Committee Chair(s)
Wraight, Colin
Committee Member(s)
  • Huang, Raven H.
  • van der Donk, Wilfred
  • Schuler, Mary
Department of Study
Biochemistry
Discipline
Biochemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
  • Chemistry
  • Biochemistry
Language
eng
Abstract
Part IV: Cleavage of 16S rRNA. Colicin E3 is a highly specific endonuclease that cleaves a single bond in 16S rRNA between A1493 and G1494. This cleavage results in termination of protein synthesis in the cell. In order to determine whether the nucleotide sequence of the target site accounts for colicin E3's specificity, we tested the activity of colicin E3 on short, synthetic double-stranded RNAs that have both identical and modified sequences of the target section of the rRNA. In addition, we tested the activity of the nuclease domain of colicin E3 on ribosomes. The results indicate that the nuclease domain of colicin E3 alone cleaves the short double stranded RNAs non-specifically, while it does not cleave the A1493-G1494 bond in rRNA of purified ribosomes.
Graduation Semester
2008
Type of Resource
text
Permalink
http://hdl.handle.net/2142/84850

Owning Collections

Graduate Dissertations and Theses at Illinois PRIMARY
Graduate Theses and Dissertations at Illinois
Dissertations and Theses - Biochemistry