Engineering T Cell Receptors for Potential Therapeutic Applications
Richman, Sarah A.
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Permalink
https://hdl.handle.net/2142/84845
Description
Title
Engineering T Cell Receptors for Potential Therapeutic Applications
Author(s)
Richman, Sarah A.
Issue Date
2007
Doctoral Committee Chair(s)
Kranz, David M.
Department of Study
Biochemistry
Discipline
Biochemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Molecular
Language
eng
Abstract
The overall goal of developing new engineering strategies to allow for the isolation of high affinity mutants of many different TCRs was extended to methods for affinity maturation in Chapter 4. Previous affinity-based selections have involved incubating yeast cells with fluorescently labeled recombinant pepMHC ligand. Many pepMHC ligands are difficult to express and purify, so an alternative affinity selection strategy that obviates the need for this material would likewise facilitate affinity maturation. The new method described in Chapter 4 selects for yeast-displayed high-affinity TCR mutants by virtue of their binding to antigen-presenting cells. The concepts and strategies described herein not only provide insight into the folding of TCR domains, but also expand the repertoire of methods for TCR engineering by yeast display.
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