University of Illinois Urbana-Champaign

The P22 Xis Protein: Regulation of Bacteriophage P22 Site -Specific Recombination

Mattis, Aras Nikodemas

This item is only available for download by members of the University of Illinois community. Students, faculty, and staff at the U of I may log in with your NetID and password to view the item. If you are trying to access an Illinois-restricted dissertation or thesis, you can request a copy through your library's Inter-Library Loan office or purchase a copy directly from ProQuest.

Permalink

https://hdl.handle.net/2142/84842

Description

Title
The P22 Xis Protein: Regulation of Bacteriophage P22 Site -Specific Recombination
Author(s)
Mattis, Aras Nikodemas
Issue Date
2007
Doctoral Committee Chair(s)
Gumport, Richard I.
Department of Study
Biochemistry
Discipline
Biochemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Language
eng
Abstract
Mutants of P22 Xis were isolated and assayed for excision system in vivo, to quantify their excision activity relative to wild-type Xis. Mutants S18F, R19A, G29S, G29D, A33P, R35C, D37G, S43F, S43P, P44L, L46F, A51T, and A51V were found to be defective in stimulating excision. Additionally, truncation mutants S66Z, K76Z, and D97Z were decreased in function about five-fold. In contrast, truncation mutants Q57Z and a mutant with the first 21 amino acids deleted were completely defective. Substitution mutants at R105, K107, and R109 revealed that this region stimulates the reactaion. A subset of Xis mutants were purified and assayed by EMSA and crosslinking studies. All purified mutants formed multimers by crosslinking, but L15F, K16E, A33P, S43F, and A55T were defective in DNA binding. Furthermore, L46F bound DNA with a weaker affinity but still formed specific complexes.
Graduation Semester
2007
Type of Resource
text
Permalink
http://hdl.handle.net/2142/84842

Owning Collections

Graduate Dissertations and Theses at Illinois PRIMARY
Graduate Theses and Dissertations at Illinois
Dissertations and Theses - Biochemistry