Characterization of the Fidelity Mechanisms of Leucyl -Trna Synthetases From Saccharomyces Cerevisiae and Escherichia Coli
Karkhanis, Vrajesh Anil
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https://hdl.handle.net/2142/84840
Description
Title
Characterization of the Fidelity Mechanisms of Leucyl -Trna Synthetases From Saccharomyces Cerevisiae and Escherichia Coli
Author(s)
Karkhanis, Vrajesh Anil
Issue Date
2007
Doctoral Committee Chair(s)
Martinis, Susan A.
Department of Study
Biochemistry
Discipline
Biochemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Language
eng
Abstract
We tested several mutations in yeast mitochondrial LeuRS that altered the post-transfer editing function of LeuRSs from other origins. Our results show that yeast mitochondrial LeuRS has maintained a competent editing active site for post-transfer editing of mischarged tRNA similar to other LeuRSs. However, unlike other origins, when LeuRS amino acid editing-defective mutants were introduced in vivo, cell viability was largely unaffected. In contrast, these editing-defective mutations limited viability of E. coli cells. We propose that the yeast mitochondria have evolved to tolerate lower levels of fidelity in protein synthesis.
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