University of Illinois Urbana-Champaign

Enzymatic and Splicing Activity of Class I Leucyl -Trna Synthetases From Escherichia Coli and Saccharomyces Cerevisiae

Boniecki, Michal Tadeusz

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Description

Title
Enzymatic and Splicing Activity of Class I Leucyl -Trna Synthetases From Escherichia Coli and Saccharomyces Cerevisiae
Author(s)
Boniecki, Michal Tadeusz
Issue Date
2007
Doctoral Committee Chair(s)
Susan Ann Martinis
Department of Study
Biochemistry
Discipline
Biochemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Language
eng
Abstract
Leucyl-tRNA synthetase from yeast mitochondria is also required for splicing of the mitochondrial bI4 and al4a introns. Splicing of these introns is dependent on coordination between ymLeuRS and bI4 maturase. An in vitro splicing assay has been established that is based on assembly of proteins into a competent ternary splicing complex. RNA deletion mutants have been quantitated for effects on complex assembly and splicing activity.
Graduation Semester
2007
Type of Resource
text
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http://hdl.handle.net/2142/84838

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