Characterization of Leucyl -Trna Synthetase From Escherichia Coli and Saccharomyces Cerevisiae in Aminoacylation, Amino Acid Editing, and Protein-Dependent RNA Splicing
Hsu, Jennifer Ling
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https://hdl.handle.net/2142/84835
Description
Title
Characterization of Leucyl -Trna Synthetase From Escherichia Coli and Saccharomyces Cerevisiae in Aminoacylation, Amino Acid Editing, and Protein-Dependent RNA Splicing
Author(s)
Hsu, Jennifer Ling
Issue Date
2006
Doctoral Committee Chair(s)
Susan Martinis
Department of Study
Biochemistry
Discipline
Biochemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Language
eng
Abstract
Using extensive deletion and site-directed mutagenesis, we characterized the C-terminal domain of LeuRSs from different origins to understand its role. The results demonstrated that the native environment of the enzyme influenced the functional role of the unique C-terminal domain of both LeuRSs. These combined biochemical investigations suggest that the yeast mitochondrial LeuRS C-terminal domain has uniquely adapted to accommodate aminoacylation, amino acid editing, and RNA-protein interactions.
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