Studies on the Proton Pumping Mechanism of Aa(3)-Type Cytochrome C Oxidase
Han, Dan
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Permalink
https://hdl.handle.net/2142/84824
Description
Title
Studies on the Proton Pumping Mechanism of Aa(3)-Type Cytochrome C Oxidase
Author(s)
Han, Dan
Issue Date
2006
Doctoral Committee Chair(s)
Gennis, Robert B.
Department of Study
Biochemistry
Discipline
Biochemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Language
eng
Abstract
The monomeric form of bovine heart mitochondrial cytochrome c oxidase was used to detect directly the proton uptake and release events during the F-to-O transition. Absorption change of the pH sensitive dye was monitored spectroscopically. Rapid proton release followed by slow proton uptake was observed. This result indicates that both the pumped protons and the protons required for chemistry at the active site might come from an internal proton pool containing at least two protons.
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