A Study of the Mechanistic Diversity Inherent to the Ribulose Phosphate Binding Barrel Superfamily
Akana, Julie A.
This item is only available for download by members of the University of Illinois community. Students, faculty, and staff at the U of I may log in with your NetID and password to view the item. If you are trying to access an Illinois-restricted dissertation or thesis, you can request a copy through your library's Inter-Library Loan office or purchase a copy directly from ProQuest.
Permalink
https://hdl.handle.net/2142/84822
Description
Title
A Study of the Mechanistic Diversity Inherent to the Ribulose Phosphate Binding Barrel Superfamily
Author(s)
Akana, Julie A.
Issue Date
2006
Doctoral Committee Chair(s)
Gerlt, John A.
Department of Study
Biochemistry
Discipline
Biochemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Language
eng
Abstract
To probe the functional plasticity of the RPBB superfamily, rational design was used to enhance the UPS activity within the KGPDC scaffold. The triple mutant E112D/T169A/R139V had an enhancement in kcat /KM by 260 fold over wild-type KGPDC. Structural studies suggest that little perturbation of the active site occurs with the 3 mutations.
Use this login method if you
don't
have an
@illinois.edu
email address.
(Oops, I do have one)
IDEALS migrated to a new platform on June 23, 2022. If you created
your account prior to this date, you will have to reset your password
using the forgot-password link below.
