Natural and Engineered Promiscuity Within the Enolase Superfamily
Millikin, Cheri Lynn
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https://hdl.handle.net/2142/84818
Description
Title
Natural and Engineered Promiscuity Within the Enolase Superfamily
Author(s)
Millikin, Cheri Lynn
Issue Date
2005
Doctoral Committee Chair(s)
Gerlt, John A.
Department of Study
Biochemistry
Discipline
Biochemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Language
eng
Abstract
The dehydration of both D-mannonate, via syn-elimination, and D-altronate, via anti-elimination, is analogous to the stereochemical promiscuity engineered into the GlucD active site. The redesign of GlucD and the characterization of RspA illustrate the same type of promiscuity: syn and anti dehydrations in the same active site with different substrates, which results from proton abstraction of stereochemically equivalent protons. These experiments illustrate how Nature could have utilized the (beta/alpha)8-barrel as a scaffold to evolve new activities.
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