Characterization of Membrane Organization and Dynamics in Giant Unilamellar Vesicles Assembled From Rat Kidney Membrane Extracts and Fragments

Cheng, Melanie Anne B.

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Description

Title

Characterization of Membrane Organization and Dynamics in Giant Unilamellar Vesicles Assembled From Rat Kidney Membrane Extracts and Fragments

Author(s)

Cheng, Melanie Anne B.

Issue Date

2005

Doctoral Committee Chair(s)

William W. Mantulin

Department of Study

Biochemistry

Discipline

Biochemistry

Degree Granting Institution

University of Illinois at Urbana-Champaign

Degree Name

Ph.D.

Degree Level

Dissertation

Keyword(s)

Biology, Cell

Language

eng

Abstract

Fluctuation correlation spectroscopy (FCS) measurements performed on the intact BBM reveal that the protein-lipid dynamics in renal BBM is complex with a large range of diffusing species. In particular, the dynamics of NaPi-IIa was related to the perturbations in the BBM as a result of dietary potassium deficiency. By calculating the diffusion coefficient of NaPi-IIa and using photon counting histogram (PCH) analysis to measure protein aggregation, we determined that the lateral diffusion of NaPi-IIa is slowed down and its aggregation is increased in potassium deficiency, both of which are associated with the decreased Na/Pi cotransport activity. This model was proposed as a means for posttranslational regulation of Na/Pi cotransport activity that is mediated by the increased sphingomyelin and glycosphingolipid content in potassium-deficient BBM, although the mechanisms by which these lipids may modulate NaPi-IIa activity have yet to be determined.

Graduation Semester

2005

Type of Resource

text

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