An Investigation of Cytochrome Bd Quinol Oxidases of Vibrio Cholerae, Rhodobacter Sphaeroides, and Salmonella Typhimurium
Thamrong-Nawasawat, Thun
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https://hdl.handle.net/2142/84807
Description
Title
An Investigation of Cytochrome Bd Quinol Oxidases of Vibrio Cholerae, Rhodobacter Sphaeroides, and Salmonella Typhimurium
Author(s)
Thamrong-Nawasawat, Thun
Issue Date
2004
Doctoral Committee Chair(s)
Gennis, Robert B.
Department of Study
Biochemistry
Discipline
Biochemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Microbiology
Language
eng
Abstract
Chapter four describes the discovery of novel members of the tri-heme family in R. sphaeroides and V. cholerae. Combining spectroscopy and activity studies with recombinant gene expression and mutagenesis, it was shown that these two organisms might harbor an oxidase that is distinct from the bd-I oxidase of E. coli . Chapter five lays out the development of a flow cytometry assay that can be used to study bacterial membrane polarization. This assay is a specific, rapid, and efficient method to screening potential ionophores and antibiotics. Chapter six utilizes the assay to investigate the mechanism of triclosan, a widely used bactericide. It was found that triclosan might disrupt bacteria's growth by directly interrupting the membrane integrity.
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