Structure and Biochemistry of RsrI Methyltransferase
Thomas, Chad Baldo
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https://hdl.handle.net/2142/84801
Description
Title
Structure and Biochemistry of RsrI Methyltransferase
Author(s)
Thomas, Chad Baldo
Issue Date
2003
Doctoral Committee Chair(s)
Gumport, Richard I.
Department of Study
Biochemistry
Discipline
Biochemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Language
eng
Abstract
Comparison of the dimer interface of M.RsrI with the recent M.MboII structure and sequence alignments allowed identification of a conserved dimerization motif. I disrupted the dimerization of M.RsrI by adding N-acetyl-phenylalanine as a competitive inhibitor and by mutation of a serine located in the dimer interface to an aspartate. Both methods resulted in inhibition of the enzyme activity, suggesting dimerization is important for enzyme activity.
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