PaaG and PaaF: New Members of the Crotonase Superfamily
Lies, Mark Andrew, III
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https://hdl.handle.net/2142/84793
Description
Title
PaaG and PaaF: New Members of the Crotonase Superfamily
Author(s)
Lies, Mark Andrew, III
Issue Date
2003
Doctoral Committee Chair(s)
Gerlt, John A.
Department of Study
Biochemistry
Discipline
Biochemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Molecular
Language
eng
Abstract
Using the published operon structure of phenylacetate utilization, BLAST searches of public sequence databases, and metabolic information from a related compound, two pathways for phenylacetate metabolism were proposed. The genes encoding PaaF, PaaG, PaaH, PaaJ, and PaaI were cloned, and the proteins expressed, and purified. I showed by enzyme assays using purified proteins that beta-oxidation in phenylacetate metabolism proceeds via adipyl-CoA intermediates. I showed that PaaG catalyzes the isomerization of trans-Delta 3-hexenedioyl-CoA to trans-Delta2-hexenedioyl-CoA, uses an Asp as a general base, and the reaction proceeds without incorporation of solvent protons.
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