University of Illinois Urbana-Champaign

Kinetic and Mechanistic Characterization of the Uracil Phosphoribosyltransferase Activity of Bacillus Subtilis PyrR

Grabner, Gail Keaton

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Description

Title
Kinetic and Mechanistic Characterization of the Uracil Phosphoribosyltransferase Activity of Bacillus Subtilis PyrR
Author(s)
Grabner, Gail Keaton
Issue Date
2002
Doctoral Committee Chair(s)
Switzer, Robert L.
Department of Study
Biochemistry
Discipline
Biochemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Language
eng
Abstract
Direct binding of ligands to PyrR was studied by equilibrium dialysis. The dissociation constant calculated for UMP was 27 +/- 3 muM, whereas the KD for PRPP was 18 +/- 2 muM. There was no detectable binding of uracil or PPi to PyrR, which was consistent with each of the four proposed conformational change models.
Graduation Semester
2002
Type of Resource
text
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http://hdl.handle.net/2142/84785

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