Estrogen Regulation of Proteinase Inhibitor 9 Gene Expression and Interleukin-1beta Production
Stobie-Krieg, Sacha Ann Skookum
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https://hdl.handle.net/2142/84779
Description
Title
Estrogen Regulation of Proteinase Inhibitor 9 Gene Expression and Interleukin-1beta Production
Author(s)
Stobie-Krieg, Sacha Ann Skookum
Issue Date
2001
Doctoral Committee Chair(s)
Shapiro, David J.
Department of Study
Biochemistry
Discipline
Biochemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Molecular
Language
eng
Abstract
The ability of several estrogens and antiestrogens to activate PI-9 gene expression was assessed by transient transfection. Of interest, the mixed agonist/antagonist tamoxifen (OHT) was an agonist on the transiently transfected reporter gene but behaved as an antagonist on the cellular gene. To begin to assess how OHT-ER interacts with the native PI-9 promoter we carded out chromatin immunoprecipitation (ChIP) assays. The ChIP assays demonstrated that moxestrol-ER complex bound to the PI-9 promoter, but that OHT-ER (and unliganded ER) did not bind. These data provide the first evidence that the agonist activity of OHT-ER stems from the inability of OHT to elicit conformational changes in chromatin which allow ER to bind to ERE's.
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