University of Illinois Urbana-Champaign

The Membrane Topology of the Microsomal Cytochromes P450: Interfacial Characterization of Protein Structure

Retzlaff, Mary Lillian Shank

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https://hdl.handle.net/2142/84494

Description

Title
The Membrane Topology of the Microsomal Cytochromes P450: Interfacial Characterization of Protein Structure
Author(s)
Retzlaff, Mary Lillian Shank
Issue Date
2000
Doctoral Committee Chair(s)
Sligar, Stephen G.
Department of Study
Chemistry
Discipline
Chemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Molecular
Language
eng
Abstract
"In order to characterize this secondary-binding interaction, a novel surface plasmon resonance technique termed ""analyte gradient-surface plasmon resonance"" (AG-SPR) has been developed and is described here. AG-SPR can be used to rapidly characterize macro-molecular interactions without requiring surface regeneration and has been used to monitor the binding of two truncated P450s with a phosphatidylcholine supported lipid bilayer. The data has been fit to a kinetic model that incorporates a semi-empirical available surface function to account for the effect of steric interactions between pre-adsorbed molecules. The results indicate that P450 does in fact contain a peripheral binding interaction. However, in order to determine an accurate binding affinity, additional factors must be incorporated into the kinetic model."
Graduation Semester
2000
Type of Resource
text
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http://hdl.handle.net/2142/84494

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