In Vitro Selection of Highly Efficient DNA Enzymes as RNA Nucleases and Metal Biosensors
Li, Jing
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https://hdl.handle.net/2142/84486
Description
Title
In Vitro Selection of Highly Efficient DNA Enzymes as RNA Nucleases and Metal Biosensors
Author(s)
Li, Jing
Issue Date
2000
Doctoral Committee Chair(s)
Lu, Yi
Department of Study
Chemistry
Discipline
Chemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Language
eng
Abstract
Based on the superior activity of 17E in Pb2+ than in other metal ions, we evaluated its potential application in sensing Pb 2+ ions. Combining the high selectivity of 17E towards Pb2+ and the high sensitivity of fluorescence detection, 17E can measure the presence of Pb2+ ions over a concentration range of three orders of magnitude, with a detection limit as low as 10 nM. This system represents the first example of DNA-based biosensor for metal ions. Similar approaches can be applied to develop deoxyribozymes that can probe other metal ions and at various concentration ranges.
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