Structural Studies on the Estrogen Receptor Ligand Binding Domain Using Electrospray Ionization Mass Spectrometry
Seielstad, Donald Arthur
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https://hdl.handle.net/2142/84430
Description
Title
Structural Studies on the Estrogen Receptor Ligand Binding Domain Using Electrospray Ionization Mass Spectrometry
Author(s)
Seielstad, Donald Arthur
Issue Date
1998
Doctoral Committee Chair(s)
Katzenellenbogen, John A.
Department of Study
Chemistry
Discipline
Chemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Organic
Language
eng
Abstract
A protocol using LC/ESI-MS and LC/ESI-MS/MS was developed and used successfully to identify the site covalent attachment of the photoaffinity label hexestrol diazirine. The application of tandem MS/MS sequencing permitted the identification of several residues near the C-terminus of the protein.
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