Engineering a Purple copper(A) Site Into the Blue Copper Protein Azurin: Construction, Spectroscopy, and Metal Substitution Studies
Hay, Michael Todd
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https://hdl.handle.net/2142/84423
Description
Title
Engineering a Purple copper(A) Site Into the Blue Copper Protein Azurin: Construction, Spectroscopy, and Metal Substitution Studies
Author(s)
Hay, Michael Todd
Issue Date
1998
Doctoral Committee Chair(s)
Lu, Yi
Department of Study
Chemistry
Discipline
Chemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Language
eng
Abstract
Metal substitution studies of azurin-CuA have been performed using various metal ions (i.e., Hg2+, Ag+, Cu +, Cd+, Rh2+, Au+, Co 2+, and Ni2+). Metal binding was determined by electronic absorption and electrospray mass spectrometry. The results obtained from theses studies indicate that there are two factors that may influence binding in azurin-CuA: (1) the site tends to bind metal combinations which produce an overall +3 charge, and (2) the site binds soft thiophilic metals. Further characterization of the above derivatives will contribute to the understanding of the metal-binding affinity, rigidity, and other structural properties of purple copper centers.
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