Top-Down Proteomics: Separation and Mass Spectral Characterization of Complex Cell Lysates From Methanosarcina Acetivorans and Human Cancer Cells
Ferguson, Jonathan T.
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https://hdl.handle.net/2142/84326
Description
Title
Top-Down Proteomics: Separation and Mass Spectral Characterization of Complex Cell Lysates From Methanosarcina Acetivorans and Human Cancer Cells
Author(s)
Ferguson, Jonathan T.
Issue Date
2008
Doctoral Committee Chair(s)
Kelleher, Neil L.
Department of Study
Chemistry
Discipline
Chemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Language
eng
Abstract
The combination of nano-LC-MS/MS with an online-offline automation platform and an iterative data search strategy has allowed for high-throughput data acquisition to be possible with top-down protein analysis. Taken together, these platforms represent the next step in increasing the utility of top-down.
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