Development and Application of Next Generation Mass Spectrometry for Protein Analysis at High-Resolution
Boyne, Michael Thomas, II
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https://hdl.handle.net/2142/84305
Description
Title
Development and Application of Next Generation Mass Spectrometry for Protein Analysis at High-Resolution
Author(s)
Boyne, Michael Thomas, II
Issue Date
2008
Doctoral Committee Chair(s)
Kelleher, Neil L.
Department of Study
Chemistry
Discipline
Chemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Analytical
Language
eng
Abstract
And finally, a high-resolution MS/MS platform was developed to analyze large peptides at >>50,000 resolving power along with a software suite for interpreting resolved isotopic distributions and handling all known protein modifications on a high throughput basis. Called Middle Down Proteomics, this process identified 7454 peptides from 2-20 kDa (1472 unique) from 555 proteins after just 23 LC-MS/MS injections. Along with greatly increased confidence for peptide identification (expectation values from 10-89 to 104) and characterization (up to 20% of peptides were detected as modified in some LC-MS/MS runs), fragmentation data with <2 ppm accuracy also enabled error tolerant and multiplexed database searching---all clearly demonstrated in this thesis.
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