Novel Applications of Top Down MS/MS for Interrogating the Human Proteome
Roth, Michael
This item is only available for download by members of the University of Illinois community. Students, faculty, and staff at the U of I may log in with your NetID and password to view the item. If you are trying to access an Illinois-restricted dissertation or thesis, you can request a copy through your library's Inter-Library Loan office or purchase a copy directly from ProQuest.
Permalink
https://hdl.handle.net/2142/84280
Description
Title
Novel Applications of Top Down MS/MS for Interrogating the Human Proteome
Author(s)
Roth, Michael
Issue Date
2007
Doctoral Committee Chair(s)
Kelleher, Neil L.
Department of Study
Chemistry
Discipline
Chemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Analytical
Language
eng
Abstract
Top Down MS analysis of proteins from human cervical carcinoma cell lines and primary human leukocytes harvested from leukoreduction filters provided identification of 261 protein forms, with ∼12% overlap between the two proteomes. The protein forms were produced by 162 unique genes (∼22% overlap). The above information demonstrates the dynamic expression patterns of proteins in various human cell types. Among the above identifications, 89 proteolysis events, 16 methylation events, 10 phosphorylation sites, 7 acetylations (non-N-terminal), and 9 other modifications were observed. These data indicate the significant post-translational complexity present in the human proteome and validate the application of Top Down in complex proteomes, with the most precise molecular characterization of human proteins demonstrated to date.
Use this login method if you
don't
have an
@illinois.edu
email address.
(Oops, I do have one)
IDEALS migrated to a new platform on June 23, 2022. If you created
your account prior to this date, you will have to reset your password
using the forgot-password link below.
