Protein -Lipid Interactions in Soluble Nanoscale Lipid Bilayers
Shaw, Andrew W.
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Permalink
https://hdl.handle.net/2142/84265
Description
Title
Protein -Lipid Interactions in Soluble Nanoscale Lipid Bilayers
Author(s)
Shaw, Andrew W.
Issue Date
2007
Doctoral Committee Chair(s)
Sligar, Stephen G.
Department of Study
Chemistry
Discipline
Chemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Language
eng
Abstract
"Finally, a method to rapidly pattern and assay Nanodiscs using microfluidics was developed. The technique uses microfluidic channels to pattern rows of Nanodiscs on a glass slide, and then deliver multiple analytes in perpendicular rows to create a ""matrix"" assay. This successfully characterized the binding of steptavidin to biotinylated lipids and annexin to phosphoserine lipids in Nanodiscs."
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