University of Illinois Urbana-Champaign

Structure and Function of Colicin E5 Ribonuclease Domain

Lin, Yi-Lun

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https://hdl.handle.net/2142/84225

Description

Title
Structure and Function of Colicin E5 Ribonuclease Domain
Author(s)
Lin, Yi-Lun
Issue Date
2006
Doctoral Committee Chair(s)
Raven Huang
Department of Study
Chemistry
Discipline
Chemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Molecular
Language
eng
Abstract
The binding of ImmE5 to ColE5 resulted in a 50% increase of fluorescence emissions. The isothermal titration calorimetry studies showed a -10 kcal/mol enthalpy change upon the formation of ColE5•ImmE5 complex. The mutational studies of ImmE5 indicated that ImmE5 K4 and D95 contributes a larger inhibitory effect toward ColE5. The structure comparison of ColE5 in homodimer and complex showed that ImmE5 not only blocked but also closed the cleft of the active site of ColE5.
Graduation Semester
2006
Type of Resource
text
Permalink
http://hdl.handle.net/2142/84225

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