Investigating Ligand Induced Conformational Changes in the Estrogen Receptor
Hurth, Kyle Marran
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https://hdl.handle.net/2142/84211
Description
Title
Investigating Ligand Induced Conformational Changes in the Estrogen Receptor
Author(s)
Hurth, Kyle Marran
Issue Date
2005
Doctoral Committee Chair(s)
Katzenellenbogen, John A.
Department of Study
Chemistry
Discipline
Chemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biophysics, General
Language
eng
Abstract
Primarily, we have investigated ER conformational structure using the reporter group method of site-directed spin labeling (SDSL), along with electron paramagnetic resonance (EPR) spectroscopy. In this work, B and C parameter calculations from EPR line-shape theory express receptor differences quantitatively as a spectrum of conformational change. Relative squared difference (RSD) analysis allows us to compare ligand receptor complexes to one another. Furthermore, a rigorous statistical analysis shows that these measurements are reproducible. Placed in context, our work helps further the development of SDSL for the examination of protein conformation, and adds significantly to the hypothesis that the ER exists in a series of dynamic conformational forms depending upon the identity of bound ligand.
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