Progress in the Synthesis and Modification of Glass Surfaces Necessary to the Study of Single Molecule Protein Folding and Nanolithography
Engebretson, David Alan
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https://hdl.handle.net/2142/84210
Description
Title
Progress in the Synthesis and Modification of Glass Surfaces Necessary to the Study of Single Molecule Protein Folding and Nanolithography
Author(s)
Engebretson, David Alan
Issue Date
2005
Doctoral Committee Chair(s)
McDonald, J. Douglas
Department of Study
Chemistry
Discipline
Chemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biophysics, General
Language
eng
Abstract
The focus of this research was to monitor the folding of a single protein molecule using sp-FRET. In order to accomplish this task, surface modification of a glass coverslip was necessary to prevent non-specific binding. Modification of the surface was accomplished by derivatizing the surface with PEG's and dextrans. Also, the surface was modified using a metal coated AFM tip, resulting in nanolithography of the surface which was observed using confocal microscopy.
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