Mass Spectrometry and Thiotemplate Assembly: Unique Insights Into the Production of Natural Products on >100 kDa Enzymes
McLoughlin, Shaun Michael
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https://hdl.handle.net/2142/84195
Description
Title
Mass Spectrometry and Thiotemplate Assembly: Unique Insights Into the Production of Natural Products on >100 kDa Enzymes
Author(s)
McLoughlin, Shaun Michael
Issue Date
2005
Doctoral Committee Chair(s)
Kelleher, Neil L.
Department of Study
Chemistry
Discipline
Chemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Language
eng
Abstract
"This work further extends initial studies into using FTMS to examine NRP/PK synthetases and provides the most detailed insights into pyochelin, yersiniabactin and FK520 biosynthesis achieved to date. This study also represents the first observation of multiple covalently modified active site peptides in parallel using robust digestion protocols. It is hoped that further refinement of this mass spectrometry-based platform will bolster NRP/PK reengineering efforts towards the design of ""unnatural"" natural products that combat modern antibiotic-resistant pathogens."
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