University of Illinois Urbana-Champaign

Development of High Pressure Surface Plasmon Resonance for the Study of Biosystems

Kelley, Jennifer Gann

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Description

Title
Development of High Pressure Surface Plasmon Resonance for the Study of Biosystems
Author(s)
Kelley, Jennifer Gann
Issue Date
2004
Doctoral Committee Chair(s)
Sligar, Stephen G.
Department of Study
Chemistry
Discipline
Chemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Analytical
Language
eng
Abstract
Through an initial set of experiments high pressure SPR (HP-SPR) was used to measure changes in the reflectivity of various liquids under hydrostatic pressures ranging from ambient to 276 bar (4000 psi). The changes in refractive index as a function of increasing pressure for these solutions were compared and an indication of how these methods can be used to determine volume changes in biomolecular interactions is provided. The application of HP-SPR was extended to the use of commercially available sensor chips. We have shown that BIAcore(TM) SA chips maintain their surface binding functionality after pressure exposure and that effects to the surface are reversible to 6000 psi. Three test systems were chosen to be studied using this instrument, each having different characteristics; cytochrome c association to cytochrome b5, unfolding of staphylococcal nuclease, and binding of the BamH1 restriction enzyme to its DNA recognition site.
Graduation Semester
2004
Type of Resource
text
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http://hdl.handle.net/2142/84153

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