Direct Analysis of Intact Proteins From Microorganisms Using Top Down Mass Spectrometry
Meng, Fanyu
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https://hdl.handle.net/2142/84127
Description
Title
Direct Analysis of Intact Proteins From Microorganisms Using Top Down Mass Spectrometry
Author(s)
Meng, Fanyu
Issue Date
2003
Doctoral Committee Chair(s)
Kelleher, Neil L.
Department of Study
Chemistry
Discipline
Chemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Analytical
Language
eng
Abstract
We applied this top down proteomics platform to study proteins from Methanococcus jannaschii and Saccharomyces cerevisiae. From the whole cell lysate of yeast, ∼120 ion species were successfully identified with 100% sequence coverage, and we also detected several post-translational modifications, including acetylations, phosphorylations, methylations, formylations and oxidations.
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