In Vitro Selection and Characterization of Transition Metal -Dependent DNAzymes and RNAzymes
Bruesehoff, Peter John
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https://hdl.handle.net/2142/84113
Description
Title
In Vitro Selection and Characterization of Transition Metal -Dependent DNAzymes and RNAzymes
Author(s)
Bruesehoff, Peter John
Issue Date
2003
Doctoral Committee Chair(s)
Lu, Yi
Department of Study
Chemistry
Discipline
Chemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Language
eng
Abstract
"These DNAzymes are dependent on Co2+ for cleavage activity. By exploiting the electronic properties of Co2+, we have used UV-Visible spectroscopy to obtain a better understanding of the metal binding properties of the selected DNAzyme systems. Through analysis of the spectroscopic and metal binding properties of these systems, in conjunction with the activity properties, a better understanding of how metals are binding in these systems has been developed. A previously selected DNAzyme system, the ""8-17"" motif, and a naturally-occurring RNAzyme system, the hammerhead motif, were also further examined in terms of the diversity of conditions under which RNA cleavage activity is supported."
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