Designing Novel Blue Copper and Purple CuA Centers in Azurin With Natural and Unnatural Amino Acids
Berry, Steven Mark
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https://hdl.handle.net/2142/84111
Description
Title
Designing Novel Blue Copper and Purple CuA Centers in Azurin With Natural and Unnatural Amino Acids
Author(s)
Berry, Steven Mark
Issue Date
2003
Doctoral Committee Chair(s)
Lu, Yi
Department of Study
Chemistry
Discipline
Chemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Language
eng
Abstract
Finally, loop-directed mutagenesis was used to incorporate a 2Fe-2S cluster binding loop from human ferrochelatase into azurin. Preliminary iron binding studies indicate the assembly of a 4Fe-4S or a 2Fe-2S iron sulfur cluster.
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